A method of recombination and agents useful for same

Methode de recombinaison et agents utiles dans ladite methode

Abstract

L'invention concerne généralement une méthode de recombinaison d'au moins deux séquences nucléotidiques, et des agents utiles dans ladite méthode. L'invention porte, plus particulièrement, sur une méthode de recombinaison, dans une cellule hôte qui exprime la recBCD nucléase ou un dérivé fonctionnel de celle-ci, d'une séquence nucléotidique avec une séquence nucléotidique linéaire. La méthode de l'invention est utile, inter alia, pour la modification de chromosomes bactériens artificiels par recombinaison homologue avec une séquence d'ADN linéaire.
The present invention relates generally to a method for the recombination of at least two nucleic acid sequences and agents useful for same. More particularly, the present invention contemplates a method of recombining, in a host cell which expresses the recBCD nuclease or a functional derivative thereof, a circular nucleic acid sequence with a linear nucleic acid sequence. The method of the present invention is useful, inter alia, for the modification of bacterial artificial chromosomes by homologous recombination with a linear DNA sequence.

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Patent Citations (1)

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NO-Patent Citations (6)

    Title
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    WO-2005010179-A1February 03, 2005The University Of Hong KongProcede de construction et de modification de grandes molecules d'adn
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