Microfluidic device for in vitro 3d cell culture experimentation

Dispositif micro-fluidique pour l'expérimentation de culture cellulaire in vitro 3d

Abstract

L'invention concerne un dispositif micro-fluidique qui permet l'expérimentation de culture cellulaire in vitro 3D et qui comprend un corps dans lequel est disposée une chambre de culture cellulaire qui est au moins partiellement remplie d'une substance support pour maintenir une culture cellulaire. Un trajet de fluide est fourni dans le corps, ledit trajet communiquant avec la chambre de culture cellulaire pour diriger un écoulement de fluide le long de la substance support. La chambre de culture au-dessus de la substance support donne sur un orifice d'accès qui est disposé au niveau d'une surface supérieure externe du corps afin de fournir un accès direct à la substance support. La substance support forme une barrière d'écoulement de fluide qui sépare le trajet de fluide de l'orifice d'accès.
A microfluidic device for in vitro 3D cell culture experimentation comprises a body in which is provided a cell culture chamber that is at least partly filled with a scaffolding substance for maintaining a cell culture. In the body a fluid path is provided that communicates with the cell culture chamber for directing a fluid stream along the scaffolding substance. The culture chamber above the scaffolding substance opens into an access port which is provided at an outer top surface of the body to provide direct access to the scaffolding substance. The scaffolding substance forms a fluid flow barrier which separates the fluid path from the access port.

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