Biosensor for and method of electrogenerated chemiluminescent detection of nucleic acid adsorbed to a solid surface

Biocapteur et procede destines a la detection par luminescence electrophotochimique d'acides nucleiques adsorbes sur une surface solide

Abstract

De l'ADN simple brin a été immobilisé sur une électrode recouverte d'une pellicule d'alcanebisphosphonate d'aluminium par immersion de cette électrode dans une solution d'ADN simple brin. Cet ADN simple brin immobilisé, marqué par Ru(bpy)32+, a été détecté par surveillance de la luminescence électrophotochimique (ECL) produite suite à l'oxydation dans une solution contenant de la tri-n-propylamine. Après l'immobilisation d'ADN simple brin non marqué, un brin d'ADN complémentaire marqué a été hybridé pour produire de l'ADN double brin sur la surface. L'étendue de l'hybridation d'ADN a été déterminée par la luminescence ECL de Ru(bpy)¿3?2+ marqué. L'ADN double brin immobilisé sur une surface pourrait également être détecté par observation de la luminescence ECL de Ru(phen)¿3?2+. La microscopie électronique à transmission (TEM) a été utilisée pour obtenir une image de la pellicule et de l'ADN immobilisé.
Single-strand DNA was immobilized on an electrode covered with an aluminum alkanebisphosphonate film by immersing it in an ss-DNA solution. The immobilized ss-DNA labeled with Ru(bpy)32+ was detected by monitoring the electrogenerated chemiluminescence (ECL) produced upon oxidation in a solution containing tri-n-propylamine. After immobilization of unlabeled ss-DNA, a complementary labeled strand DNA was hybridized to produce ds-DNA on the surface. The extent of DNA hybridization was determined by ECL of the labeled Ru(bpy)¿3?2+. Surface immobilized ds-DNA could also be detected by observing the ECL of intercalated Ru(phen)¿3?2+. Transmission electron microscopy (TEM) was employed to image the film and the immobilized DNA.

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